| 在大肠杆菌中高效表达人类免疫缺陷病毒Ⅰ型(HIV-1)核心蛋白 |
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| 引用本文： | 吴卫星,张维,马贤凯. 在大肠杆菌中高效表达人类免疫缺陷病毒Ⅰ型(HIV-1)核心蛋白[J]. 病毒学报, 1991, 0(3) |
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| 作者姓名： | 吴卫星 张维 马贤凯 |
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| 作者单位： | 军事医学科学院基础医学研究所，北京市第二传染病医院，军事医学科学院基础医学研究所 北京，北京 |
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| 摘 要： | 将编码人类免疫缺陷病毒Ⅰ型(HIV-1)核心蛋白(Gag)P24的大部分基因片段,插入大肠杆菌表达载体pEX31A中,获得的表达产物MS2-Gag融合蛋白占菌体总蛋白的20％左右。Western blot结果证明,这种表达产物能被艾滋病(AIDS)患者血清中的特异性抗体所识别。这是国内首次报道在大肠杆菌中高效表达人类免疫缺陷病毒Ⅰ型核心蛋白。对表达的重组核心蛋白的意义及应用前景进行了讨论。
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| 关 键 词： | 人类免疫缺陷病毒Ⅰ型 核心蛋白P24 大肠杆菌 高效表达 Western blot ELISA |
HIGH LEVEL EXPRESSION OF HIV-1 GAG-P24 PROTEIN IN E. COLI |
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| Wu Weixing Zhang Wei Ma Xiakai. HIGH LEVEL EXPRESSION OF HIV-1 GAG-P24 PROTEIN IN E. COLI[J]. Chinese journal of virology, 1991, 0(3) |
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| Authors: | Wu Weixing Zhang Wei Ma Xiakai |
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| Abstract: | Gag-P24 is a major protein during the maturation of HIV-1 virion. A gene segment encoding Gag-P24 was cloned on pEX31, and Gag-P24 protein were expressed as MS2-Gag-P24 fusion protein under the control of PL promoter in E. coli. This represents about 20% of total bacterial proteins. Western blot and ELISA showed that the expressed product can be recognized by specific anti-P24 antibody in AIDS patients serum. This is the first demonstration of high level expression of Gag-P24 protein of HIV-1 in our country. The significance of the expressed recombinant Gag-P24 protein is discussed. |
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| Keywords: | HIV-1 Gag-P24 protein E. coli High levelexpression Western blot ELISA |
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