Ultrastructural study of the life cycle of <Emphasis Type="Italic">Rickettsia slovaca</Emphasis>, wild and standard type,cultivated in L929 and vero cell lines |
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Authors: | V Boldi? J ?trus E Kocianová M Tu?ek-?nidari? K ?tefanidesová E ?pitalská |
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Institution: | (1) Institute of Virology, Slovak Academy of Sciences, 845 05 Bratislava, Slovakia;(2) Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia |
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Abstract: | Ultrastructural changes induced by Rickettsia slovaca standard type (ST) and wild type (WT) were examined during their life cycle in L929 and Vero cells. R. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. Rickettsiae adhering to the cytoplasmic membrane
were engulfed by cellular extensions and occurred in phagocytic vacuoles. Binary fission of rickettsia was observed. The nuclear
chromatin of eukaryotic cells was rearranged and condensed during 3rd and 6th d p.i. Finally, loss of the plasma membrane
integrity, destruction of cytoplasm and nucleus resulted in cell lysis. Degeneration of the host cell caused by WT and ST
was observed after 4 and 5 d p.i. in L929 cells and after 3 and 6 d p.i. in Vero cells, respectively. WT type was able to
penetrate into the nucleus of the host cell and was responsible for dilatation of the perinuclear space and endoplasmic reticulum,
causing more pronounced and different cytopathological changes than the ST. |
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