Affiliation: | aLaboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, CZ-142 20 Prague 4, Czech Republic bInstitute of Chemical Technology, CZ-166 28 Prague, Czech Republic cBlaise Pascal University, Laboratory SEESIB, UMR 6504 CNRS, F-63 177 Aubière Cedex, France dMEGA, CZ-471 27 Stráž pod Ralskem, Czech Republic |
Abstract: | Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles, LentiKats®. The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylenebutanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was most stable during the transformation of (R,S)-3-hydroxy-2-methylenebutanenitrile. No significant loss of the amidase activity was observed within the course of the biocatalytic reaction. |