Interaction of syntenin-1 and the NG2 proteoglycan in migratory oligodendrocyte precursor cells |
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Authors: | Chatterjee Nivedita Stegmüller Judith Schätzle Philipp Karram Khalad Koroll Michael Werner Hauke B Nave Klaus-Armin Trotter Jacqueline |
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Institution: | Molecular Cell Biology, Department of Biology, Johannes Gutenberg University of Mainz, Mainz, Germany. |
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Abstract: | Migration of oligodendrocyte precursors along axons is a necessary prerequisite for myelination, but little is known about underlying mechanisms. NG2 is a large membrane proteoglycan implicated in oligodendrocyte migration. Here we show that a PDZ domain protein termed syntenin-1 interacts with NG2 and that syntenin-1 is necessary for normal rates of migration. The association of syntenin-1 with NG2, identified in a yeast two-hybrid screen, was confirmed by colocalization of both proteins within processes of oligodendroglial precursor cells and by coimmunoprecipitation from cell extracts. Syntenin-1 also colocalizes with NG2 in "co-capping" assays, demonstrating a lateral association of both proteins in live oligodendrocytes. RNA interference-mediated down-regulation of syntenin-1 in glial cells results in a significant reduction of migration in vitro, as does the presence of polyclonal antibody against NG2. Thus syntenin plays a role in the migration of oligodendroglial precursors, and we suggest that NG2-syntenin-1 interactions contribute to this. |
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