Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli |
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| Authors: | Zhu Fen; Suzuki Kengo; Okada Kazunori; Tanaka Katsunori; Nakagawa Tsuyoshi; Kawamukai Makoto; Matsuda Hideyuki |
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| Institution: | 1 Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University Matsue, 690 Japan
2 Research Institute of Molecular Genetics, Shimane University Matsue, 690 Japan |
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| Abstract: | A gene encoding a novel geranylgeranyl pyrophosphate (GGPP)synthase from Arabidopsis thaliana has been identified and termedGGPS5. The gene has been sequenced and expressed in Escherichiacoli. The deduced amino acid sequence showed 64.5% and 57.5%identity with a putative GGPP synthase from Arabidopsis andCapsicum annuum, respectively. GGPP enzymatic activity was detectedin E. coli cells expressing the GGPS5 gene in two differentways. One was the direct measurement of GGPP synthase activityin cell extracts and the other was the yellow color productionof cells when the GGPS5 gene was co-expressed with crtB, crtI,crtX, crtY and crtZ genes derived from Erwinia uredovora. (Received May 20, 1996; Accepted December 14, 1996) |
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