猫须草抗炎活性成分研究

为了研究猫须草(Clerodendranthus spicatus)抗炎活性成分,该研究采用活性追踪的方法,利用硅胶、MCI柱色谱以及HPLC等分离技术,对猫须草抗炎活性部位进行分离纯化,通过波谱数据分析和文献比对鉴定化合物结构,并利用LPS诱导的RAW 264.7巨噬细胞炎症模型进行抗炎活性评价。结果表明:(1)从猫须草抗炎活性部位分离得到10个化合物,分别鉴定为泡桐素(1)、鼠尾草素(2)、对苯二甲酸二辛酯(3)、N-(N-苯甲酰基-L-苯丙酰基)-L-苯基丙醇(4)、fragransin B₁(5)、6,7,8,4''-四甲氧基黄酮(6)、N-反式-阿魏酰酪胺(7)、N-顺式-阿魏酰酪胺(8)、trans-N-cinnamoyltyramine(9)、新海胆灵 A(10),其中化合物1、4、8-10为首次从该植物中分离得到。(2)抗炎结果显示,猫须草抗炎活性成分主要存在于中低极性部位,从中分离得到的大部分化合物显示出一定的NO生成抑制活性,其中酰胺类成分(7-9)均具有较好的抗炎活性,表明该类成分是猫须草抗炎作用的主要成分之一。该研究丰富了猫须草抗炎物质基础,为其开发利用提供科学依据。

Anti-inflammatory constituents of Clerodendranthus spicatus

To study the anti-inflammatory constituents of Clerodendranthus spicatus. With the aid of activity tracing, the compounds were isolated from the anti-inflammatory active fractions of C. spicatus through various separation methods including column chromatography on silica gel, MCI and HPLC. Their chemical structures were identified by analyzing their spectra data and comparing with published literatures. The inflammatory cell model, built by LPS-induced RAW 264.7 macrophage cells, was used to evaluate anti-inflammatory effects. The results were as follows:(1)Ten compounds were obtained from anti-inflammatory active fractions and their structures were identified as paulownin(1), salvigenin(2), bis-(2-ethylhexyl)terephthalate(3), N-(N-benzoy-L-phenylalany)-L-phenylalanol(4), fragransin B₁(5), 6,7,8,4''-tetramethoxyflavone(6), N-trans-feruloyltyramine(7), N-cis-feruloyltyramine(8), trans-N-cinnamoyltyramine(9), neoechinulin A(10), respectively. Compounds 1, 4, 8-10 were obtained from this plant for the first time.(2)The anti-inflammatory test results showed that the anti-inflammatory constituents of C. spicatus mainly existed in the mid-low polarity extract. Most of compounds showed certain inhibition effects on NO production. Among them, amides(7-9)also showed better anti-inflammatory effects, indicating that amides are one of main anti-inflammatory constituents of C. spicatus. This study enriches the material basis of anti-inflammatory of C. spicatus, and also provides a scientific basis for its development and utilization.