The isolation and characterization of ngm2, a mutation that affects nitrosoguanidine mutagenesis in yeast |
| |
Authors: | Paul E Nisson and Christopher W Lawrence |
| |
Institution: | (1) Department of Radiation Biology and Biophysics, University of Rochester, School of Medicine and Dentistry, 14642 Rochester, NY, USA |
| |
Abstract: | Summary We have isolated and characterized a new mutant of Saccharomyces cerevisiae, carrying a single mutant allele that we designate ngm2-1, which is defective with respect to induced mutagenesis. This mutant was isolated by screening mutagenized clones for reduced frequencies of reversion of the his1-7 allele, induced by N-methyl-N-nitro-N-nitrosoguanidine. As judged by the reversion of his1-7 and ilv1-92, ngm2-1 mutant strains are also deficient with respect to mutability induced by methyl methane sulfonate, ethyl methane sulfonate and, at least partially, by UV. UV-induced reversion of the ochre mutation arg4-17 and the frameshift mutation his4-38 was not much affected by ngm2-1, however. Like rev3 and rev7 mutations, ngm2-1 also has little influence on the reversion of the proline missense allele, cyc1-115. Ngm2-1 mutants are only at best very slightly more sensitive to the toxicity of the four mutagens used, and homozygous diploids sporulate normally. |
| |
Keywords: | Yeast N-methyl-N-nitro-N-nitrosoguanidine" target="_blank">gif" alt="prime" align="BASELINE" BORDER="0">-nitro-N-nitrosoguanidine Induced mutagenesis New mutant Chemical mutagenesis |
本文献已被 SpringerLink 等数据库收录! |
|