Immunocytochemical localization of serine: pyruvate aminotransferase in peroxisomes of the human liver parenchymal cells |
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Authors: | S Yokota T Oda A Ichiyama |
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Institution: | Department of Anatomy, Yamanashi Medical School, Japan. |
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Abstract: | The localization of serine:pyruvate aminotransferase (SPT) in human liver was investigated by indirect immunoenzyme and protein A-gold techniques. By light microscopy, diaminobenzidine reaction product was present in cytoplasmic granules of the parenchymal cells. By electron microscopy, gold particles indicating the antigenic sites for SPT were exclusively confined to peroxisomes but not to mitochondria. By double labeling technique, both peroxisomal marker enzyme, catalase and SPT were detected in the same peroxisomes. Quantitative analysis of the labeling density showed that SPT is contained only in peroxisomes. The results indicate that in human liver most of SPT is contained in the peroxisomes. |
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