Extracellular pH affects regulation of the pcbAB gene in Penicillium chrysogenum |
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| Authors: | Y-W Chu D Renno G Saunders |
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| Institution: | (1) University of Westminster, School of Biological and Health Sciences, 115 New Cavendish Street, London W1M 8JS, UK. Fax: +44 181 886 0687 e-mail saundeg@wmin.ac.uk, GB |
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| Abstract: | The effect of extracellular pH and dissolved oxygen on regulation of the pcbAB gene in P.␣chrysogenum was examined, using Northern analysis and a reporter gene fusion. It was found that ambient pH markedly affected levels of
pcbAB mRNA whereas maintenance of dissolved oxygen concentration above 10 % had no detectable effect. The presence of a DNA-binding
protein, which binds upstream of the pcbAB translational start codon, was also related to ambient pH. In all fermentations, pcbAB mRNA was most abundant at around the late exponential/early stationary phase of a culture.
Received: 10 May 1996 / Received revision: 14 October 1996 / Accepted: 25 October 1996 |
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