Somatic embryogenesis and regeneration of flowering plants in rose |
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| Authors: | Joyce C de Wit Harald F Esendam Jarmo J Honkanen Ulla Tuominen |
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| Institution: | (1) Terra Nigra B. V., Iepenlaan 48, 1424 PA De Kwakel, Holland;(2) Kemira Oy, Espoo Research Centre, P.O. Box 44, SF-02271 Espoo, Finland |
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| Abstract: | Summary Somatic embryos of the cut rose cultivars  Domingo and Vickey Brown were obtained from callus derived from leaf explants on half strength Murashige and Skoog medium with low concentrations of kinetin and 1-naphthyl acetic acid or 2-naphthyloxyacetic acid. Somatic embryos were first observed after 6 to 12 weeks of culture on callus formed at the basis or midrib of the leaf. Embryos could be grown to phenotypically true to type greenhouse plants.Abbreviations IAA
3-indole-acetic acid
- IBA
indole-3-butyric acid
- NAA
1-naphthylacetic acid
- NOA
2-naphthyloxyacetic acid
- BAP
6-benzylaminopurine
- KIN
6-furfurylaminopurine
- MS
Murashige and Skoog |
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