A novel family of linear plasmids with homology to plasmid pAL2-1 of Podospora anserina |
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Authors: | Josef Hermanns Fons Debets Rolf Hoekstra Heinz D. Osiewacz |
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Affiliation: | (1) German Cancer Research Center, Department of Molecular Biology of Aging Processes, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany;(2) Wageningen Agricultural University, Wageningen, The Netherlands;(3) Present address: Biozentrum Niederursel, AK Molekulare Enwicklungsbiologie und Biotechnologie, Johann Wolfgang Goethe Universität, D-60439 Frankfurt am Main, Germany |
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Abstract: | Three recently isolated wild-type strains of the ascomycete Podospora anserina were analyzed for the presence of linear mitochondrial plasmids. In one of these strains, designated Wa6, at least 12 distinct plasmid-like elements were identified. From molecular analyses a minimum number of 78 individual linear molecules with proteins bound to their 5 ends was estimated. In addition, the different members of this family of typical linear plasmids were shown to possess a common central region and terminal sequences which differ from one plasmid to another due to the presence of different numbers of a 2.4 kb sequence module. Finally, the pWa6 plasmids share a high degree of sequence similarity with pAL2-1, a linear plasmid previously identified in mitochondria of a long-lived mutant of P.anserina. A mechanism is proposed which explains the generation of these distinct, closely related extrachromosomal genetic traits. |
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Keywords: | Podospora anserina Linear plasmids Mitochondrial DNA |
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