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Synthesis of the nascent strand of tRNAfMet from E coli. |
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| Authors: | E Ohtsuka T Tanaka S Tanaka K Fujiyama A F Markham E Nakagawa T Wakabayashi Y Taniyama S Nishikawa R Fukumoto H Uemura T Doi M Ikehara |
| Abstract: | Oligonucleotides corresponding to the total tRNAfMET FROM E. coli have been synthesized. These fragments were joined by using RNA ligase to yield quarter molecules. The 5'-quarter molecule showed 84% amino acid acceptor activity when it was combined with the natural three quarter molecule. |
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