| 两种裸藻的无叶绿体突变株 |
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| 引用本文： | 王江新,施之新,徐旭东. 两种裸藻的无叶绿体突变株[J]. 水生生物学报, 2002, 26(2): 175-179 |
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| 作者姓名： | 王江新 施之新 徐旭东 |
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| 作者单位： | 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 中国科学院“百人计划”项目资助 |
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| 摘 要： | 利用Ofloxacin处理获得了纤细裸藻和中型裸藻无叶绿体突变株。吸收光谱测定和自荧光显微观察证明突变株无叶绿素合成，DAPI染色方法显示突变株细胞不存在质体DNA，而无色的长变胞藻细胞中仍有可检测质体DNA，说明二突变株质体已消失。SDS－PAGE显示野生种和突变株各有特异蛋白表达。
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| 关 键 词： | 裸藻 无叶绿体突变株 质体 |
| 文章编号： | 1000-3207(2002)02-0175-005 |
| 修稿时间： | 2001-07-10 |
CHLOROPLAST-LESS MUTANTS OF TWO SPECIES OF EUGLENA |
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| WANG Jiang xin,SHI Zhi xin and XU Xu dong. CHLOROPLAST-LESS MUTANTS OF TWO SPECIES OF EUGLENA[J]. Acta Hydrobiologica Sinica, 2002, 26(2): 175-179 |
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| Authors: | WANG Jiang xin SHI Zhi xin XU Xu dong |
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| Abstract: | Chloroplast less mutants of E gracilis and E intermedia were generated by treatment with Ofloxacin Absorption spectra and autofluorescence microscopic observations showed that chlorophyll was not synthesized in the two mutants Using DAPI fluorescence photomicrograph, plastid DNA was found in Astasia longa , a colorless species of Euglenoid, but not in the ofloxacin induced mutants of Euglena, which suggest that plastids have been completely lost in the two mutants SDS PAGE indicated that specific proteins were synthesized in the wild type and mutant strains respectively |
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| Keywords: | Euglena Chloroplast Mutants |
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