Changes in isotopic signatures of soil carbon and CO2 respiration immediately and one year after Miscanthus removal

The removal of perennial bioenergy crops, such as Miscanthus, has rarely been studied although it is an important form of land use change. Miscanthus is a C4 plant, and the carbon (C) it deposits during its growth has a different isotopic signature (¹²/¹³C) compared to a C3 plant. Identifying the proportion of C stored and released to the atmosphere is important information for ecosystem models and life cycle analyses. During a removal experiment in June 2011 of a 20‐year old Miscanthus field (Grignon, France), vegetation was removed mechanically and chemically. Two replicate plots were converted into a rotation of annual crops, two plots had Miscanthus removed with no soil disturbance, followed by bare soil (set‐aside), one control plot was left with continued Miscanthus cultivation, and an adjacent field was used as annual arable crops control. There was a significant difference in the isotopic composition of the total soil C under Miscanthus compared with adjacent annual arable crops in all three measured soil layers (0–5, 5–10 and 10–20 cm). Before Miscanthus removal, total C in the soil under Miscanthus ranged from 4.9% in the top layer to 3.9% in the lower layers with δ¹³C values of ?16.3 to ?17.8 while soil C under the adjacent arable crop was significantly lower and ranged from 1.6 to 2% with δ¹³C values of ?23.2. This did not change much in 2012, suggesting the accumulation of soil C under Miscanthus persists for at least the first year. In contrast, the isotopic signals of soil respiration 1 year after Miscanthus removal from recultivated and set‐aside plots were similar to that of the annual arable control, while just after removal the signals were similar to that of the Miscanthus control. This suggests a rapid change in the form of soil C pools that are respired.