Nitrogen removal performance and microbial community structure dynamics response to carbon nitrogen ratio in a compact suspended carrier biofilm reactor |
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| Authors: | Siqing Xia Junying Li Rongchang Wang |
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| Institution: | 1. School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;2. Basic Research Service, Ministry of Science and Technology of the People’s Republic of China, Beijing 100862, China;3. State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150090, China;4. School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;1. Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing, PR China;2. National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing, PR China;3. Xian University Architecture & Technology, Int Sci & Technol Cooperat Ctr Urban Alternat Wat, Key Lab Northwest Water Resource Environm & Ecol, MOE, Engn Technol Res Ctr Wastewater Treatment & R, 13 Yanta Rd, Xian 710055, Shanxi, PR China;1. Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, PR China;2. Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun 130118, PR China;3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Environmental and Municipal Engineering, Bioprocess Centre For Sustainable Environment, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Jinjing Road 26, Tianjin 300384, China;2. Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NWS 2007, Australia |
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| Abstract: | A compact suspended carrier biofilm reactor (SCBR) was developed for simultaneous nitrification and denitrification (SND) in a single reactor and the performance of nutrient removal was investigated. Microbial community structure response to different ratio of carbon to nitrogen (C/N) was determined by denaturing gel gradient electrophoresis (DGGE) profiles of 16S rDNA V3 region and amoA gene amplifications. In addition, the population dynamics of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were estimated by fluorescence in situ hybridization (FISH) with 16S rDNA-targeted oligonucleotide probes. Results showed that the compact SCBR was efficient in nutrient removal with CODCr removal efficiency over 90% and SND efficiency (ESND) about 83.3%. The diversity of microbial community structure was positively correlated with C/N ratio, while the three communities of amoA gene were relativity homogenous. The population of nitrifiers was in inverse proportions to C/N ratio with the average fraction of AOB and NOB to all bacteria 5.4, 4.8, 3.1% and 4.6, 3.5, 2.7% respectively as C/N ratio changing from 3:1, 5:1 to 10:1. Therefore we could reach a conclusion that the compact SCBR was practical to treat municipal wastewater and the shift of microbial community monitored by molecular technologies could offer guidance to the process optimization in engineering. |
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