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Influence of plant species and wastewater strength on constructed wetland methane emissions and associated microbial populations
Authors:Yanhua Wang  Ryuhei Inamori  Hainan Kong  Kaiqin Xu  Yuhei Inamori  Takashi Kondo  Jixiang Zhang
Affiliation:1. School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, PR China;2. Faculty of Symbiotic Systems Science, Fukushima University, 1 Kanayagawa, Fukushima 960-1296, Japan;3. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan;4. State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan Unviversity, Wuhan 430072, PR China;5. School of Economics and Management, Southeast University, Nanjing, Jiangsu, 210096, PR China;1. Key Laboratory of Agro-ecological Processes in Subtropical Regions, Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, PR China;2. University of Chinese Academy of Sciences, Beijing, 100039, PR China;3. Faculty of Life Science and Technology, Central South University of Forestry and Technology, Hunan, 410004, PR China;1. Key Laboratory of Agro-ecological Processes in Subtropical Regions, Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Geography, Institute of Ecology and Earth Sciences, University of Tartu, 46 Vanemuise Street, 51014 Tartu, Estonia;2. Hydrosystems and Bioprocesses Research Unit, National Research Institute of Science and Technology for Environment and Agriculture (Irstea), 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France;3. Everglades Wetland Research Park, Florida Gulf Coast University, 4940 Bayshore Drive, Naples, FL 34112, USA;1. Institute of Ecology and Earth Sciences, University of Tartu, 51014 Tartu, Estonia;2. Hydrosystems and Bioprocesses Research Unit, National Research Institute of Science and Technology for Environment and Agriculture (Irstea), 1 rue Pierre-Gilles de Gennes CS 10030, F92761 Antony cedex, France;3. Thünen Institute of Climate-Smart Agriculture, 38116 Braunschweig, Germany;4. UPMC Univ Paris 06, Centre National de la Recherche Scientifique, IEESP, 4 Place Jussieu, 75252 Paris cedex 05, France
Abstract:Wastewater and treatment processes have been regarded as large contributions to sources of methane (CH4). The flux rate of CH4 in constructed wetlands (CWs) was evaluated to test the influence of plant species. Methane emission data showed large temporal and spatial variation ranging from 0 to 16.76 g CH4 m−2 day−1. The highest CH4 flux rate was obtained in the Zizania latifolia systems and higher emission was found with higher influent load. The methanogenic and methanotrophic microbial populations were studied to clarify the mechanisms of CH4 emission. FISH analysis showed highest amounts of methanogens and methanotrophs in the Z. latifolia and Tytha latifolia systems. Linear regression between CH4 emission and environmental parameters showed that the regression lines were not forced to pass through the origin, and the slopes of the lines of different systems were allowed to vary between vegetation cover.
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