| 具有高外切葡聚糖纤维二糖水解酶Ⅰ活力的新型黄单胞工程菌的构建 |
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| 引用本文： | 王春卉,汪天虹,高培基,钟玲WANG Chun-Hui,WANG Tain-Hong,GAO Pei-Ji,ZHONG Ling.具有高外切葡聚糖纤维二糖水解酶Ⅰ活力的新型黄单胞工程菌的构建[J].遗传,1997,19(5):37-40. |
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| 作者姓名： | 王春卉 汪天虹 高培基 钟玲WANG Chun-Hui WANG Tain-Hong GAO Pei-Ji ZHONG Ling |
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| 作者单位： | 山东大学生命科学学院微生物系,济南 250100
Microbiology Department,The college of Life Science,Shandong University,Jinan 250100 |
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| 摘 要： | 由广宿主质粒pRK404和微紫青霉外切葡聚糖纤维二糖水解酶Ⅰ(CBHI)c DNA基因cbhⅠ构建了重组质粒pRK404-181,在携带有诱动质粒的菌株ED86 54(pRK2013)的存在下, 采用三亲滤膜结合的方法,将该质粒转移到野油菜黄单胞菌S-152中,并成功地得到表达。结合子S-152(pRK404-181)外切葡聚糖纤维二糖水解酶的pNPC活力比原始菌株S-152提高了2-4倍,滤纸酶活提高了1倍。
Abstract:Recombinant plasmid pRK404-181 was constructed with the wide-host-range plasmid pRK404 and the cellubiohydrolase I(cbh I)gene of Penicillium janthinellum.The plasmid was transferred into Xanthomonas campestris S-152 with the aid of helper plasmid pRK2013.As a result,the intracellular collubiohydrolase activity of S-152(pPK404-181)increased 2~4 time,and the filter paper degrading activity increased 1 time in comparison with the initial bacterial Xanthomonas campestris S-152.
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| 关 键 词： | 接合转移 微紫青霉 Conjugation 外切葡聚糖纤维二糖水解酶基因 Key words 野油菜黄单胞菌 亚型 马铃薯 classⅠpatatin基因 块茎 |
Construction of Novel Engineering Xanthomonas with Higher Cellubiohydrolase I Activity |
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| WANG Chun-Hui,WANG Tain-Hong,GAO Pei-Ji,ZHONG Ling.Construction of Novel Engineering Xanthomonas with Higher Cellubiohydrolase I Activity[J].Hereditas,1997,19(5):37-40. |
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| Authors: | WANG Chun-Hui WANG Tain-Hong GAO Pei-Ji ZHONG Ling |
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| Abstract: | |
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| Keywords: | Subtype Tuber class I patatin gene Potato |
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