| 昆明山海棠在微核实验中非整倍体毒性的研究 |
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| 引用本文： | 曹佳,胡斌,程天民,程舸. 昆明山海棠在微核实验中非整倍体毒性的研究[J]. 遗传, 1997, 19(1): 1-3 |
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| 作者姓名： | 曹佳 胡斌 程天民 程舸 |
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| 作者单位： | 1.第三军医大学预防医学系, 重庆 6300382.广州师范学院生物系，广州市5104001.Preventive Medicine Department,Third Military Medical University,Chongqing 6300382.Biology Department,Guangzhou Teachers′ College,Guangzhou 510400 |
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| 基金项目： | 国家自然科学基金,总后回国启动基金 |
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| 摘 要： | 本文报道以小鼠着丝粒次要卫星DNA探针FISH和抗着丝粒CREST染色,研究了可疑的非整倍体毒剂昆明山棠(THH)诱导的小鼠NIH3T3细胞微核(MN)的着丝粒组成情况,结果THH(10-60μg/ml)诱导的62.1-68.4%的MN为FISH阳性, 35.9-42.2%的MN为CREST阳性,较精确的FISH结果显示THH具有较强的非整倍体诱发效应,同时认为次要卫星DNA探针比CREST染色更适合于MN的着丝粒检测。
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| 关 键 词： | 非整倍体 着丝粒 微核 昆明山海棠 区带特异性 pUC19文库 微小卫星DNA (CA)n |
The Study on the Aneuploidy Induced by Tripterygium hypoglaucum(Level)Hutch in Micronucleus Test |
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| CHAO Jia,HU Bin,CHENG Tian-Min,Cheng Ke. The Study on the Aneuploidy Induced by Tripterygium hypoglaucum(Level)Hutch in Micronucleus Test[J]. Hereditas, 1997, 19(1): 1-3 |
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| Authors: | CHAO Jia HU Bin CHENG Tian-Min Cheng Ke |
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| Abstract: | The fluorescence in situ hybridization(FISH) with murine minor satellite DNA probe and antikinetochore antibodies(CREST staining)techniques were applied to study the kinetochore compositions of micronuclei(MN)induced by a suspect aneugen Tripterygium hypoglaucum(Level) Hutch(THH) in mouse NIH3T3 fibroblast cell lines.The results showed the labeling frequencies of MN with the FISH and CREST were identical 62.1~68.4% and 36.9~42.2% respectively.The precise results of FISH indicated that THH has stronger aneuploidy toxicity.Comparision of the results between the CREST staining and FISH,the minor satellite DNA probe is more suitable to detect the kinetochores in MN. |
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| Keywords: | Tripterygium hypoglaucum (Level) Hutch Micronuclei Aneuploidy Kinetochore |
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