High frequency adventitious shoot regeneration in sainfoin |
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Authors: | Özgen Murat Özcan Sebahattin Sevimay Cafer S Sancak Cengiz Yildiz Mustafa |
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Institution: | (1) Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110 Dişkapi- Ankara, Turkey |
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Abstract: | A procedure is described for the rapid and efficient adventitious shoot regeneration from leaflets, petioles and stems of
field-grown sainfoin plants. All explants formed shoots on a range of media supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic
acid (NAA). Stem explants appeared to have better regeneration capacity than leaflet and petiole explants in most media tested.
The highest frequency of shoot regeneration was achieved from stem segments on a medium containing 20 μM BA and 0.5 μM NAA.
Regenerated shoots rooted in half-strength Murashige and Skoog medium containing 5 μM indole-3-butyric acid and later established
well under greenhouse conditions.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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