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Screening for Fusarin C production by European isolates of Fusarium species
Authors:Ulf Thrane
Institution:1. Department of Biotechnology, Building cx221, The Technical University of Denmark, DK-2800, Lyngby
2. the Biotechnical Section of the Engineering Academy of Denmark, DK-2800, Lyngby, Denmark
Abstract:A total of 137Fusarium isolates were screened forin vitro production of the mutagenic metabolite fusarin C, using a simple thin layer chromatographic method. It has been proven thatFusarium species (F. culmorum, F. graminearum, F. crookwellense, F. sporotrichioides, F. poae, F. tricinctum, andF. Avenaceum) isolated from European agricultural crops and soils are able to produce fusarin C. No fusarin C production was detected among isolates ofF. arthrosporioides, F. acuminatum, or F. equiseti. Results obtained by High-Performance Liquid Chromatography (HPLC) analyses of fungal extracts show that up to 26 chromatographic peaks having UV spectra similar to that of fusarin C are produced. It is not known if any of these metabolites are as mutagenic as fusarin C.
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