Affinity chromatography as a rapid and convenient method for purification of fungal laccases |
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| Authors: | Rogalski J Wojtas-Wasilewska M Apalovic R Leonowicz A |
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| Affiliation: | Department of Biochemistry, Maria Curie-Sk?odowska University, 20-031 Lublin, Poland. |
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| Abstract: | A rapid and convenient method for graduation, isolation, and purification of laccase from Trametes versicolor and Fomes fomentarius culture fluids was developed. For purification affinity chromatography on syringyl- and vanillyl-controlled porosity glass (CPG) columns was applied. The purified laccase of F. fomentarius was immobilized on porous glass. Some properties of the immobilized enzyme in comparison to the free one are discussed. |
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| Keywords: | laccase Trametes versicolor Fomes fomentarius affinity chromatography purification |
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