Inorganic phosphate determination in the presence of a labile organic phosphate: assay for carbamyl phosphate phosphatase activity |
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Authors: | M J Black M E Jones |
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Affiliation: | 1. Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, Minnesota 55455 USA;2. Department of Biochemistry, University of Minnesota, Minneapolis, Minnesota 55455 USA |
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Abstract: | A periodate-resorcinol method for bound sialic acid using the Technicon Autoanalyzer II is presented. It has a sensitivity similar to the manual method, is linear between 5 and 65 nmol/ml, requires less than 0.2 ml of sample, and can be run at the rate of 70 samples/h. Little cross-reaction with common matrix and cell components was found. The method is compatible with many commonly used volatile and nonvolatile chromatographic buffers. The use of a bound sialic acid standard such as N-acetylneuraminyl lactose is recommended. |
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Keywords: | carbamyl phosphate phosphatase orthophosphate assay |
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