Plasmodesmata transport of GFP alone or fused to potato virus X TGBp1 is diffusion driven |
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Authors: | G Schönknecht J E Brown J Verchot-Lubicz |
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Institution: | (1) Botany Department, Oklahoma State University, Stillwater, Oklahoma;(2) Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma |
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Abstract: | Summary. Plasmodesmata (Pd) provide a pathway for exchanging various macromolecules between neighboring plant cells. Researchers routinely
characterize the mobility of the green-fluorescent protein (GFP) and GFP fusions through Pd by calculating the proportion
of sites in bombarded leaves which show fluorescence in multiple cell clusters (% movement). Here, the Arrhenius equation
was used to describe the temperature dependence of GFP and GFP-TGBp1 (potato virus X triple gene block protein1) movement,
using % movement values, and to calculate the activation energy for protein transport. The resulting low activation energy
indicates GFP and GFP-TGBp1 movement are diffusion driven. Furthermore, GFP movement is inversely proportional to the leaf
surface area of expanding leaves. The increase in leaf area results mainly from cell expansion during the sink–source transition.
The increasing cell size results in lower Pd density, which decreases the probability that a GFP attains an open Pd by diffusion.
The decline in GFP movement as leaf area expands indicates that, in addition to GFP diffusion through Pd, attaining an open
Pd by undirected diffusion might be limiting for Pd transport. In summary, this report provides a new quantitative method
for studying Pd conductivity.
Correspondence: Jeanmarie Verchot Lubicz, Department of Entomology and Plant Pathology, 127 Noble Research Center, Oklahoma
State University, Stillwater, OK 74078, U.S.A. |
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Keywords: | : Plasmodesmata transport Green-fluorescent protein Viral movement protein Potato virus X Diffusion Intercellular transport |
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