High-Efficiency Rescue of Equine Infectious Anemia Virus from a CMV-Driven Infectious Clone |
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| Authors: | Wang Xue-Feng Bai Bowen Lin Yuezhi Qi Ting Du Cheng Song Mingxin Wang Xiaojun |
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| Institution: | 1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China2.State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150069, China |
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| Abstract: | In this study, the strong promoter of CMV was introduced in place of the 5' LTR U3 of EIAV to improve the expression level of the molecular clone and to promote the rescue efficiency of the virus. We demonstrated that the EIAV infectious clone pCMV3-8 have a strong capacity to rescue EIAV by viral RT activity assay, Western blotting and TEM. Taken together, these results indicate that pCMV3-8 could be used for genetic manipulation of EIAV and can be applied to the researches on the mechanism of viral replication and attenuated EIAV vaccine. |
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| Keywords: | , , |
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