Regeneration of haploid and dihaploid plants from protoplasts of supersweet (sh2sh2) corn |
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| Authors: | C S Sun L M Prioli M R Söndahl |
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| Institution: | (1) DNA Plant Technology Corporation, 2611 Branch Pike, 08077 Cinnaminson, NJ, USA;(2) Present address: Institute of Botany, Academia Sinica, Beijing, China |
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| Abstract: | Summary Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures. |
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| Keywords: | Zea mays L supersweet corn protoplasts haploids plant regeneration |
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