A new astrocytic cell line which is able to induce a blood-brain barrier property in cultured brain capillary endothelial cells |
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Authors: | Carsten T Beuckmann Katja Dernbach Ansgar Hakvoort Hans-Joachim Galla |
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Institution: | Institut für Biochemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Straße 2, D-48149 Münster, F.R, Germany |
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Abstract: | Astrocytes, a member of the glial cell family in the central nervous system, are assumed to play a crucial role in the formation
of the blood-brain barrier (BBB) in vertebrates. It was shown that astrocytes induce BBB-properties in brain capillary endothelial
cells (BCEC) in vitro. We now established an astroglial cell line of non-tumoral origin. The cloned cell line (A7) shows a
highly increased proliferation rate and expresses the astrocytic marker glial fibrillary acidic protein. Furthermore, the
clone A7 expresses S-100-protein and vimentin, which are also expressed by primary cultured astrocytes. This cell line therefore
shows general astrocytic features. In addition, we were able to show that A7 cells re-induce the BBB-related marker enzyme
alkaline phosphatase in BCEC, when these two cell types are co-cultured. Thus we have a cell line which can be readily cultured
in large quantities, shows common astrocyte properties and is able to influence BCEC with respect to a BBB-related feature.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | astrocytes blood-brain barrier clone continuous cell line |
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