In vivo kinetics and biodistribution of a Hantaan virus DNA vaccine after intramuscular injection in mice |
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Authors: | Si Wang Qing Nie Lan-yan Zheng Jun Hu En-jie Luo |
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Institution: | Department of Medical microbiology and parasitology, Preclinical Medical College, China Medical University, Shenyang 110001, China |
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Abstract: | To study the kinetics in vivo of a Hantaan virus DNA vaccine, we constructed a fusion DNA vaccine,pEGFP/S, by cloning the S segment of Hantavirus into the vector, pEGFP-C1, which encodes Green fluorescent protein EGFP. In this report, we provide evidence that pEGFP/S was distributed and persistently expressed for more than 60 days in several organs after inoculation. Our findings suggest that the persistent immune responses induced by a Hantaan virus DNA vaccine are likely due to the plasmid pEGFP/S deposited in vivo, which acts as a booster immunization. |
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Keywords: | Hantaa virus DNA vaccine Intramuscular injection Immunologic memory Nucleocapsid protein |
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