Glucocorticoids modulate mRNA levels for G-protein beta-subunits. |
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| Authors: | M Ros D C Watkins P J Rapiejko and C C Malbon |
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| Institution: | Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651. |
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| Abstract: | Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein beta-subunits (G beta) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G beta 5.8 +/- 0.4 amol/micrograms of RNA (n = 5) in control fat-cells]. G beta mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G beta mRNA levels by 50%. |
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