New polymorphic markers in the vicinity of the pearl locus on mouse Chromosome 13 |
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| Authors: | H. -P. Xu B. L. Yanak M. H. Wigler M. B. Gorin |
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| Affiliation: | (1) Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, New York 11724, USA, US;(2) Department of Ophthalmology, The Eye and Ear Institute of Pittsburgh, University of Pittsburgh, 203 Lothrop Street, Pittsburgh, Pennsylvania 15213, USA, US;(3) Department of Human Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA, US |
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| Abstract: | We have used a Mus domesticus/-Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the four markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory. Received: 12 June 1995 / Accepted: 17 August 1995 |
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