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Development of new microsatellite markers from a salt-marsh sedge Carex rugulosa by compound simple sequence repeat-polymerase chain reaction |
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| Authors: | Ohbayashi K Hodoki Y Nakayama S Shimada M Kunii H |
| Affiliation: | Research Center for Coastal Lagoon Environments, Shimane University, 1060 Nishi-Kawatsu-Cho, Matsue, Shimane 690-8504, Japan, Graduate School of Science, The University of Tokyo, 1842 Hanaishi, Nikko, Tochigi 321-1435, Japan, Department of System Sciences (Biology), The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan. |
| Abstract: | We developed 12 polymorphic microsatellite markers from a salt-marsh sedge Carex rugulosa. The number of alleles per locus ranged from two to four, with an average of 2.75. The observed and expected heterozygosities ranged from 0.067 to 0.600 and from 0.128 to 0.620, respectively. These simple sequence repeat markers will allow the identification of genets and evaluation of the genetic diversity of C. rugulosa. |
| Keywords: | Carex rugulosa compound microsatellite marker salt‐marsh sedge SSR |
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