Polymorphic microsatellite markers for the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae), a vector of human African trypanosomiasis |
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| Authors: | Brown J E Komatsu K J Abila P P Robinson A S Okedi L M A Dyer N Donnelly M J Slotman M A Caccone A |
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| Institution: | Department of Ecology and Evolutionary Biology, Yale University, 21 Sachem Street, New Haven, CT 06511, USA, National Livestock Resources Research Institute, PO Box 96, Tororo, Uganda, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Insect Pest Control Sub-Programme, International Atomic Energy Agency, A-1400 Vienna, Austria, Vector Research Group, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK. |
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| Abstract: | Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has been severely constrained by a lack of genetic markers for mapping and population genetic studies. Here we present 10 newly developed microsatellite loci for this tsetse species. Heterozygosity levels in Moyo, an Ugandan population, averaged 0.57, with only two loci showing very low heterozygosity. Five loci carried more than six alleles. Together with five recently published microsatellite loci, this brings the number of available microsatellite loci for this species to 15. Their availability will greatly facilitate future studies on the genetics of this important human disease vector. |
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| Keywords: | genetic marker Glossina fuscipes microsatellite loci sleeping sickness tsetse |
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