Characterization of single nucleotide polymorphism markers for eelgrass (Zostera marina) |
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| Authors: | Ferber Steven Reusch Thorsten B H Stam Wytze T Olsen Jeanine L |
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| Affiliation: | Department of Marine Benthic Ecology and Evolution, Centre for Ecological and Evolutionary Studies, University of Groningen, Kerklaan 30, PO Box 14, 9750 AA Haren, the Netherlands Plant Evolutionary Ecology, Institute for Evolution and Biodiversity, University of Münster, D48149 Münster, Germany. |
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| Abstract: | We characterized 37 single nucleotide polymorphism (SNP) makers for eelgrass Zostera marina. SNP markers were developed using existing EST (expressed sequence tag)-libraries to locate polymorphic loci and develop primers from the functional expressed genes that are deposited in The ZOSTERA database (V1.2.1). SNP loci were genotyped using a single-base-extension approach which facilitated high-throughput genotyping with minimal optimization time. These markers show a wide range of variability among 25 eelgrass populations and will be useful for population genetic studies including evaluation of population structure, historical demography, and phylogeography. Potential applications include haplotype inference of physically linked SNPs and identification of genes under selection for temperature and desiccation stress. |
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| Keywords: | expressed sequence tag high‐throughput seagrass single nucleotide polymorphism SNaPshot Zostera marina |
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