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Isolation and characterization of microsatellite markers in Oligoryzomys longicaudatus (Muridae, Sigmodontinae, Oryzomini), the natural reservoir of genotype Andes hantavirus
Authors:González-Ittig Raúl E  Salazar-Bravo Jorge  Polop Jaime J  Gardenal Cristina N
Institution:Cátedra de Genética de Poblaciones y Evolución, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, 5000 Córdoba, Argentina, Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409-3131, USA, Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Agencia Postal No. 3, 5084 Río Cuarto, Córdoba, Argentina.
Abstract:The rodent Oligoryzomys longicaudatus or long-tailed pygmy rice rat is the reservoir of the aetiological agent of the hantavirus pulmonary syndrome in southern Argentina and Chile. We characterize 11 polymorphic microsatellite loci which would be useful for studies on microgeographical population structure in the species. Amplification of these loci in 42 individuals from four natural populations revealed four to 21 alleles per locus, and values of observed heterozygosities ranging from 0.371 to 0.896. Cross-species amplifications showed that some of the primers designed may be useful for other species of the genus Oligoryzomys.
Keywords:hantavirus pulmonary syndrome  microsatellites  Oligoryzomys longicaudatus
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