Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross-species amplification in other Heroini cichlid species |
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| Authors: | Amado Manuella Villar Hrbek Tomas Gravena Waleska Fantin Cleiton DE Assunção Enedina Nogueira Astolfi-Filho Spartaco Farias Izeni P |
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| Institution: | 1. Departamento de Biologia, Laboratório de Evolu??o e Genética Animal, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jord?o Ramos, 3000, 69077‐000, Manaus, Amazonas, Brazil,;2. Biology Department, University of Puerto Rico ‐ Rio Piedras, San Juan, PR 00931, Puerto Rico,;3. Centro de Apoio Muldisciplinar, Laboratório de Tecnologias de DNA, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jord?o Ramos, 3000, 69077‐000, Manaus, Amazonas, Brazil |
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| Abstract: | The discus fishes (Symphysodon spp.) are economically important ornamental species. Thirteen microsatellite markers were developed from a CT(12) - and CA(12) -enriched whole genomic DNA library of Symphysodon discus. Allelic variability was tested on 44 individuals of two species (S. discus and S. aequifasciatus). Allelic richness ranged from two to 11 alleles per locus and observed heterozygosities from 0.083 to 0.998. All loci were at Hardy-Weinberg equilibrium, and no pair of loci showed linkage disequilibrium within a species. Cross-species amplification was also successfully performed in the Neotropical cichlids Uaru amphiacanthoides, Hoplarchus psittacus, Hypselecara coryphaenoides, Pterophyllum sp., Mesonauta sp. and Heros sp. |
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| Keywords: | Amazonia Cichlidae discus microsatellites ornamental fish Symphysodon |
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