Isolation and characterization of 10 microsatellite loci in Callicarpa subpubescens (Verbenaceae), an endemic species of the Bonin Islands |
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| Authors: | Mori K Kaneko S Isagi Y Murakami N Kato H |
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| Institution: | Makino Herbarium, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan, Graduate School of International Development and Cooperation, Hiroshima University, Kagamiyama 1-5-1, Higashi-Hiroshima 739-8529, Japan, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan. |
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| Abstract: | Ten microsatellite loci were isolated and characterized for Callicarpa subpubescens (Verbenaceae), an endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from two to eight with an average of 4.9, and the expected heterozygosity ranged from 0.238 to 0.690 with an average of 0.483. All 10 loci were screened in cross-amplification tests for two other endemic Callicarpa species that also inhabit the Bonin Islands. All loci were successfully amplified in these species. |
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| Keywords: | Bonin (Ogasawara) Islands cross‐amplification gene flow microsatellite SSR markers Verbenaceae |
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