纳米载体介导的植物遗传转化研究现状和前景

高效遗传转化技术是植物重要性状功能基因鉴定的前提和转基因育种的基础.随着纳米生物技术的发展,以纳米载体介导的植物转基因技术已显示出巨大的应用潜力.综述了国内外应用于植物纳米载体的类型、与外源基因的结合方式以及传输细胞的原理,重点阐述了影响纳米基因载体性能与转化效率的重要因素,以及纳米载体介导外源基因转化植物细胞的方法,...

Research Progress and Prospect of Plant Genetic Transformation Mediated by Nano-gene Vector

Efficient genetic transformation technology is the prerequisite for the identification of functional genes for important traits in plants and the basis for transgenic breeding.With the development of nanotechnology,the genetic transformation technology of plant based on gene vector constructed by nano particle has shown great application potential.The type and properties of nano particle applied in gene vector of plant,its combination way with DNA and the basic principle of gene transfection technology were reviewed.In the meanwhile,the important factors that affect the performance and transformation efficiency of nano-gene vector,as well as the transformation methods by which nano-gene vectors mediate exogenous genes into plant cells,were expounded emphatically.The advantages of nano-gene vectors were analyzed compared with that of other vectors.Further researches are needed to improve the stable genetic transformation,gene editing and in planta transformation with nano-gene vector,which will provide new ideas for plant genetic transformation technology and methods.