| miR-378促进脂质生成相关靶基因鉴定 |
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| 引用本文： | 张廷焕,龙熙,郭宗义,柴捷. miR-378促进脂质生成相关靶基因鉴定[J]. 生物技术通报, 2021, 0(2): 80-87 |
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| 作者姓名： | 张廷焕 龙熙 郭宗义 柴捷 |
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| 作者单位： | 重庆市畜牧科学院;农业部养猪科学重点实验室 |
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| 基金项目： | 重庆市科研院所绩效激励引导专项(19536);国家自然科学基金面上项目(31771376);重庆市畜牧科学院基本科研业务费专项项目(15428)。 |
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| 摘 要： | 旨为验证miR-378在脂肪细胞中的功能,及其脂质相关靶基因的筛选和鉴定.利用miR-378类似物转染3T3-L1细胞,验证miR-378在脂肪细胞中的功能;根据靶标位点的保守性以及促脂功能确定miR-378潜在靶基因;采用microRNA pulldown技术验证miR-378与靶基因的靶标关系;运用双荧光素报告基因...
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| 关 键 词： | miR-378 脂质生成 microRNA pulldown 靶基因 靶位点 |
miR-378 Promoting Lipogenesis and Identification of Target Genes |
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| ZHANG Ting-huan,LONG Xi,GUO Zong-yi,CHAI Jie. miR-378 Promoting Lipogenesis and Identification of Target Genes[J]. Biotechnology Bulletin, 2021, 0(2): 80-87 |
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| Authors: | ZHANG Ting-huan LONG Xi GUO Zong-yi CHAI Jie |
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| Affiliation: | (Chongqing Academy of Animal Science,Chongqing 402460;Key Laboratory of Pig Industry Sciences of Ministry of Agriculture,Chongqing 402460) |
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| Abstract: | The purpose of this study was to investigate the function of miR-378 in adipocytes and to filter and verify its target genes.3T3-L1 cells were transfected with miR-378 mimic to validate the function of mir-378 in adipocytes.The potential target genes of miR-378 were identified according to the conservation of target sites and lipid promoting function.The relationship between miR-378 and its targets was verified by microRNA pulldown technology,and then the binding sites of miR-378 and its targets were determined by Dual-Luciferase Reporter Assay System.Some important results were found that miR-378 promoted lipogenesis in adipocytes by increasing lipid synthesis and decreasing lipid decomposition,and the relationship between miR-378 and Runx1t1,Galnt3,RAB10 and the binding sites were determined. |
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| Keywords: | miR-378 lipogenesis microRNA pulldown target genes target sites |
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