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In vitro culture of Cuminum cyminum regeneration of flowering shoots from calli of hypocotyl and leaf explants
Authors:Timir Baran Jha  Satyesh Chandra Roy  Gopal Chandra Mitra
Institution:(1) Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700 019 Calcutta, India
Abstract:Calli from hypocotyl explant of Cuminum cyminum L. (Cumin) grew rapidly on Gamborg's B5 basal medium with the following supplements, (i) 0.5 mg/l — 2,4-D (ii) 4 mg/l — NAA plus 2 mg/l — Kinetin and (iii) 0.2 mg/l — NAA plus 0.2 mg/l — BAP, whereas calli from leaf explant in these media grew slowly. Hypocotyl and leaf calli produced roots when transferred to basal medium only and shoots in basal medium with 0.5 mg/l NAA and 0.1 mg/l BAP. Ninety percent of the shoots produced roots when they were transferred to half strength MS inorganic salts supplemented with 0.5 mg/l each of IBA and NAA.Fifty to sixty percent of rootless as well as rooted shoots produced terminal umbellate flowers on this medium.
Keywords:Cuminum cyminum  hypocotyl and leaf calli  rooted shoots  flowering  transplantable plantlets
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