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Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry |
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| Authors: | Wenfei Zhang Jie Zhang Neil Crickmore Zhongqi Wu Yiran Yang Jiangzhao Qian Hongping Wu Ruiping Wang Xuanjun Fang |
| Affiliation: | 1. Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China 2. School of Life Sciences, University of Sussex, Falmer, Brighton, UK 3. Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, 572025, Hainan, China |
| Abstract: | The Bacillus thuringiensis strain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea, cry30 Ga, cry50Ba and cry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30 kDa. In this study we used mass spectrometry to identify the 130 kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity against Culex quinquefasciatus. |
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