重组人血管内皮抑制素（rh-Endostatin）大肠杆菌表达体系发酵条件的优化

优化了重组人血管内皮抑制素的E. coli表达体系的发酵条件。利用E. coli表达体系得到了较高的产量，在9h左右的发酵周期内达到OD600值140，包涵体蛋白产量为3 g/L。主要优化了异丙基-β-D-硫代半乳糖苷（Isopropyl-β-D-thiogalactopyranoside, IPTG）的终浓度、诱导时间、培养温度、补料控制方法等条件，并且在诱导后提高培养温度到40℃，在非常短的培养周期内达到了高密度培养的目的。利用E. coli表达，继而通过复性获得有活性的重组人血管内皮抑制素，成本低、生产过程稳定可控、得到的蛋白性质稳定，符合工业生产的需要。

Optimization of Fermentation of Recombinant Human Endostatin (rh-Endo-statin) Expression in Escherichia coli

The fermentation process of recombinant human Endostatin expression in Escherichia coli BL21 (DE3) was studied. The effects of factors such as concentration of IPTG, induction time, cultivation temperature and feeding strategies were investigated. Beside that, by changing the temperature to 40 degrees C after induction, the high-density cultivation finished in a much shorter period. After 9 hours cultivation, the optical density (OD) at 600 nm reached 140 and the yield of inclusion body was 3 g/L. While E. coli system was used, protein with better activity and stability was obtained. The cost was much lower and the producing process was much steadier. It will meet the demands of the industrial production.