Characterization of six microsatellite loci in Myrica faya (Myricaceae) and cross amplification in the endangered endemic M. rivas-martinezii in Canary Islands, Spain |
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Authors: | González-Pérez Miguel A Newton Craig Sosa Pedro A Rivero Elizabeth González-González Edna A |
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Affiliation: | Departamento de Biología, Universidad de Las Palmas de Gran Canaria, Canary Islands Spain. |
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Abstract: | Six novel polymorphic microsatellite markers were isolated from enriched libraries in Myrica faya Ait., recently renamed Morella faya, (fayatree, firetree, or firebush) in order to examine the genetic diversity in natural populations. Also, test cross-specific amplification and genetic diversity in Myrica rivas-martinezii, which is endemic on the Canary islands. Microsatellite loci were screened in 225 individuals of both species from different islands of the Canarian archipelago. All markers were successfully amplified from both Myrica species, with an average number of 6.5 and 9.3 alleles per locus in M. rivas-martinezii and M. faya, respectively. There was no evidence for linkage disequilibrium between loci, and the probability of null alleles ranged from 0.01 to 0.17. |
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Keywords: | Myrica rivas-martinezii Myrica faya microsatellite Canary Islands genetic diversity |
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