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Karyological study of Amphisbaena ridleyi (Squamata, Amphisbaenidae), an endemic species of the Archipelago of Fernando de Noronha, Pernambuco, Brazil
Authors:Laguna Marcia Maria  Amaro Renata Cecília  Mott Tamí  Yonenaga-Yassuda Yatiyo  Rodrigues Miguel Trefaut
Institution:1.Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil;2.Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil;3.Instituto de Biociências, Programa de Pós Graduação em Ecologia e Conservação da Biodiversidade, Universidade Federal do Mato Grosso, Cuiabá, MT, Brazil
Abstract:The karyotype of Amphisbaena ridleyi, an endemic species of the archipelago of Fernando de Noronha, in State of Pernambuco, Brazil, is described after conventional staining, Ag-NOR impregnation and fluorescence in situ hybridization (FISH) with a telomeric probe. The diploid number is 46, with nine pairs of macrochromosomes (three metacentrics, four subtelocentrics and two acrocentrics) and 14 pairs of microchromosomes. The Ag-NOR is located in the telomeric region of the long arm of metacentric chromosome 2 and FISH revealed signals only in the telomeric region of all chromosomes. Further cytogenetic data on other amphisbaenians as well as a robust phylogenetic hypothesis of this clade is needed in order to understand the evolutionary changes on amphisbaenian karyotypes.
Keywords:Amphisbaena ridleyi  karyotype  Fernando de Noronha  Ag-NOR  FISH with telomeric probes
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