| 固相小柱对沙苑子总黄酮萃取方法的研究 |
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| 引用本文： | 牟英,彭少平,顾振纶. 固相小柱对沙苑子总黄酮萃取方法的研究[J]. 中国野生植物资源, 2006, 25(6): 55-57 |
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| 作者姓名： | 牟英 彭少平 顾振纶 |
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| 作者单位： | 苏州中药研究所,苏州大学基础医学系药理教研室,江苏,苏州,215000 |
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| 摘 要： | 目的:以沙苑子总黄酮为研究对象,考察固相萃取小柱的洗脱条件,确定固相萃取沙苑子总黄酮的实验方法。方法:以不同浓度的甲醇对不同填料的固相小柱进行洗脱,将洗脱液在岛津VP-ODS(150 mm×4.6 mm,5μm)柱上进行高效液相色谱分析。通过比较不同浓度及体积甲醇的萃取效率,确定沙苑子苷在固相萃取小柱上的洗脱条件。结果:用正相氨基小柱以50%甲醇洗脱效果好,回收率可达100%。结论:经试验,正相氨基小柱对沙苑子苷的萃取回收率约为100%,适用于沙苑子总黄酮中沙苑子苷的分析。
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| 关 键 词： | 固相萃取 沙苑子总黄酮 |
| 文章编号： | 1006-9690(2006)06-0055-03 |
| 修稿时间： | 2006-02-20 |
Study on the Extraction Method of Total Flavonoids of Astragalus complanatus R.Br.by Solid Phase Cartridges |
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| Mou Ying,Peng Shaoping,Gu Zhenlun. Study on the Extraction Method of Total Flavonoids of Astragalus complanatus R.Br.by Solid Phase Cartridges[J]. Chinese Wild Plant Resources, 2006, 25(6): 55-57 |
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| Authors: | Mou Ying Peng Shaoping Gu Zhenlun |
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| Abstract: | Objective:To optimize the solid phase extraction method with total flavonids in Astragalus complanatus R.Br.Mothod:After extracted by different sokd phase cartridges with different content method elution,the elutin was analyzed on a Shimadzu VP-ODS HPLC column.From the recovery achived,the suitable content and volume of elution was found.Result:This method consists of using 10 mL of 50%methanol as the elutin.Between the two kinds of solid phase being tested,normal phase cartridge was found to be the best one.Conclusion:The average extraction recovery of the normal phase cartridges was about 100%.It can be used in the analysis of total flavonoids in Astragalus complanatus R.Br. |
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| Keywords: | HPLC |
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