High-Pressure Plastic Lamination for Flexible Mounts of Cover-Slip Preparations

A Carver laminating press has been used for the lamination of small glass cover slip preparations of smears, aspiration biopsies, organ imprints and tissue culture materials between 41/2 × 57/8 × 0.012 inch thick Vinylite plastic sheets at 10,000 lb/inch² of pressure and 400° F. The procedure described was to minimize some of the difficulties encountered in the cytological assessment of a variety of tissue and cell preparations. Lamination flattens the cells sufficiently to increase cytological detail and to reduce overlapping without sacrificing staining properties. Giemsa, Wright's methylene blue, orcein, and hematoxylin-eosin stains were used in the preparations. None of these stains were altered by the procedure. Laminated cell preparations are more suitable for making automatic and visual cell counts and for counting intracellular organoids