犬瘟热病毒细胞膜受体的鉴定

犬瘟热病毒（ＣＤＶ）敏感细胞Ｖｅｒｏ用ＳＤＳ或ＲＩＰＡ溶解缓冲液溶解，利用病毒铺覆蛋白印迹技术（ＶＯＰＢＡ）鉴定犬瘟热病毒疫苗株（ＣＤＶ－ｏｎｄｅｓｔｅｐｏｏｒｔ）的细胞受体。结果发现，在Ｖｅｒｏ细胞上有两组ＣＤＶ结合蛋白质，即高分子量组蛋白质（１２７ｋＤ、１２０ｋＤ、１１０ｋＤ）与低分子量组蛋白质（２７ｋＤ和３０ｋＤ）。这些ＣＤＶ结合蛋白组分的性质及在ＣＤＶ致病中的作用有等进一步研究。

Identifitation of Canine Distemper Virus Receptor in Cell Lines

The cell membrane from permissive Vero cells was solubilized by SDS or RIPA lysis buffer, and then subjected to the virus overlay protein blot assay (VOPBA) to identify CDV binding proteins. The results showed that there were two groups of cellular protein that could bind CDV in Vero cells, one with high molecular masses of 127kD, 120kD, 110kD and the other with relatively low molecular masses of 27kD, 30kD. The characteristics of these proteins remain to be studied further.