Improvement of enterocin P purification process |
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Authors: | S Cuozzo S Calvez H Prévost D Drider |
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Institution: | Laboratoire de Microbiologie Alimentaire et Industrielle, Ecole Nationale des Ingénieurs des Industries des Techniques Agricoles et Alimentaires, BP 82225 Nantes, France. |
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Abstract: | Purification and heterologous expression of enterocin P (EntP), a sec-dependent bacteriocin produced by Enterococcus faecium, in Escherichia coli is described. PCR-amplified product of the enterocin P structural gene entP was cloned into plasmid pET-32b under the control of the inducible T7lac promoter. The neo-synthesized EntP was genetically modified by an addition of 3 extra amino acids, leading to recombinant EntRP. Active EntRP was recovered from the cytoplasmic soluble fraction of E. coli harboring appropriate recombinant plasmid, characterized by ELISA and Western-blot analysis and purified by immunoaffinity chromatography. The use of E. coli as heterologous host and pET-32b as expressing vector offers promising tools for heterologous production of class IIa bacteriocin. |
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