基于稳定同位素比率及营养差异的双孢蘑菇不同栽培基质的溯源分析

双孢蘑菇是世界消费量最大的食用菌之一,不同于欧美国家的麦草栽培配方,我国双孢蘑菇栽培材料主要是以水稻秸秆为主.为了降低农业秸秆废弃物的焚烧和我国双孢蘑菇产业的持续发展,需因地制宜地开发和利用各地特色农业废弃物资源作为双孢蘑菇栽培的基质.为鉴别不同材料栽培的双孢蘑菇子实体的营养和产量差异,本研究以8种不同基质配方栽培的双...

Origin traceability analyses of different cultivation substrate of Agaricus bisporus based on stable isotope ratio and nutrient difference

Agaricus bisporus is one of the edible fungi with the world’s largest consumption. Being different from using wheat straw as substrate in European countries, rice straw was used as the main substrate for A. bisporus cultivation in China. In order to reduce burning agricultural straw waste and sustain development of A. bisporus industry, it is necessary to develop and utilize the agricultural waste resources as the substrate for A. bisporus cultivation in the light of local conditions. It is well known that the nutrition and yield of fruiting bodies of different cultured substrate are significantly different. The stable isotope analysis is widely used in tracing studies of agricultural products. In this study, based on eight substrate formulations with different nutritional components and proportions, the stable isotope ratios of the cultured A. bisporus were compared and determined. The results showed that the content of cellulose, calcium, zinc and amino acid in eight different formulation were significantly different and the four stable isotope ratio of δ¹³C, δ¹⁵N, δ²H and δ¹⁸O studies showed significant differences. Principal component analysis (PCA) of 4 different nutrient factors and 4 isotope ratios showed that the A. bisporus cultivated on different substrate could be effectively divided into three categories, and the contribution rate of the three principal components was 78.2%, indicating that the grouping was explained better. The analysis of stable isotope and fruiting body nutrition is possibly used as approaches to grade and trace the A. bisporus industrial products.