基于1H-NMR及GC-MS的中国块菌及其共生云南松的代谢物分析

采用代谢组学技术研究中国块菌(云南黑松露)及其共生云南松的小分子活性代谢产物.运用核磁共振(1H-NMR)和气相质谱(GC-MS)两种技术,分析新鲜中国块菌成熟子实体及松根的代谢成分;将1H-NMR及GC-MS数据导入SMICA-P软件进行多元统计分析,比较二者的差异代谢物.在块菌子实体及松根中分别共检测到了苏氨酸、甘...

Analyses of metabolites of Tuber sinense and its symbiotic Pinus yunnanensis based on ~1H-NMR and GC-MS

Small molecule active metabolites of Tuber sinense (Yunnan black truffle) and its symbiotic Pinus yunnanensis were studied by metabonomics technique. Nuclear magnetic resonance (¹H-NMR) and gas phase mass spectrometry (GC-MS) were used to analyze the metabolic components of mature fruit-body of fresh Tuber sinense and pine root. The ¹H-NMR and GC-MS data were imported into SMICA-P software for multivariate statistical analysis, and the differential metabolites between them were compared. A total of 64 and 45 compounds, including threonine, mannobiose, fumaric acid, theophylline and epicatechin, was detected in truffle and pine roots, respectively, among which, 25 compounds, such as terpenol, 1-cyclohexene-1-formic acid, glutamate, trehalose and betaine, showed significant differences, and these differential compounds were mainly related to carbon metabolism and nitrogen metabolism. Mutual complement of the two methods can expand the detection range. This study provides a reference for exploring the symbiotic mechanism of Tuber sinense and its symbiotic pine and developing Tuber sinense related drugs and artificial culture in future.