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Periodicity of response to abscisic acid in lateral buds of willow (Salix viminalis L.)
Authors:R. S. Barros  S. J. Neill
Affiliation:(1) Department of Botany and Microbiology, University College of Wales, SY23 3DA Aberystwyth, Dyfed, UK;(2) Present address: Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570 Viçosa, Minas Gerais, Brazil
Abstract:Aseptically cultured lateral buds of Salix viminalis L. collected from field-grown trees exhibited a clear periodicity in their ability to respond to exogenous abscisic acid (ABA). Buds were kept unopened by ABA only when the plants were dormant or entering dormancy. Short days alone did not induce bud dormancy in potted plants but ABA treatment following exposure to an 8-h photoperiod prevented bud opening although ABA treatment of buds from long-day plants did not. Naturally dormant buds taken from shoots of field-grown trees and cultured in the presence of ABA opened following a chilling treatment. In no cases were the induction and breaking of dormancy and response to ABA correlated with endogenous ABA levels in the buds.Abbreviations ABA abscisic acid - GA3 gibberellic acid - HPLC high-performance liquid chromatography - LD long day - MeABA methyl ABA - PAR photosynthetically active radiation - SD short day
Keywords:Abscisic acid (periodic response to)  Bud (lateral)  Chilling  Dormancy  Photoperiod, ABA and bud dormancy  Salix
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