Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy |
| |
Authors: | A Grziwa W Baumeister B Dahlmann F Kopp |
| |
Affiliation: | Max-Planck-Institut für Biochemie, Martinsried, Germany. |
| |
Abstract: | The subunit topography of the Thermoplasma acidophilum proteasome was determined by iminunoelectron microscopy using monospecific antibodies directed against the two constituent subunits (,β). Anti--subunit IgG was found to bind to the outer disks of the cylinder- or barrel-shaped molecule, while the binding sites of the anti-β-subunit IgG were mapped on the two inner rings. Probably the homologues of the two subunits in the compositionally more complex but isomorphous eukaryotic proteasomes occupy equivalent positions. |
| |
Keywords: | Proteasome Multicatalytic proteinase Archaebacterium Immunoelectron microscopy |
本文献已被 ScienceDirect 等数据库收录! |